Why does Chromatographs of sequenced DNA usually has broad peaks in the begining?
is it because it is hard to sequence when there is only a base pairs?
Answer:
Usually the beginning and the end of the sequence is pretty bad. The middle of the sequence is fine. I beleve I could blame this occurance on an over shot of DNA into the instrument. Try selecting another primer that could cover the beginning and/or the end of the sequence.
good luck.
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Answer:
Usually the beginning and the end of the sequence is pretty bad. The middle of the sequence is fine. I beleve I could blame this occurance on an over shot of DNA into the instrument. Try selecting another primer that could cover the beginning and/or the end of the sequence.
good luck.
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