Anyone know the significance of avidin and biotin in spectroscopy?
I'm a research member of FTIR (Fourier Transform Infrared Spectroscopy) team and we do self-assembled monolayer composed of avidin and biotin.
I've been diggin up some information about avidin/biotin but I'm very confused about its significance to infrared spectroscopy.
What are their functions? what's so significant about their characteristics? and what effect do they have as self-assembled monolayer?
more answer = better for me so please help me!!
Answer:
Biotin is most often used as a marker for molecules to separate them out from a mixture of various other substances. Avidin (as well as steptavidin) and biotin have a high affinity. This allows avidin to be placed on solid support (in the form of beads) on a column and then the mixture is put on the column. All of the molecules that are not biotinylated are washed away and the molecules with biotin in them can be eluted with an appropriate substance. Most likely, your team uses this to identify molecules.
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I've been diggin up some information about avidin/biotin but I'm very confused about its significance to infrared spectroscopy.
What are their functions? what's so significant about their characteristics? and what effect do they have as self-assembled monolayer?
more answer = better for me so please help me!!
Answer:
Biotin is most often used as a marker for molecules to separate them out from a mixture of various other substances. Avidin (as well as steptavidin) and biotin have a high affinity. This allows avidin to be placed on solid support (in the form of beads) on a column and then the mixture is put on the column. All of the molecules that are not biotinylated are washed away and the molecules with biotin in them can be eluted with an appropriate substance. Most likely, your team uses this to identify molecules.
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