What happens when you have bubbles in a polarimeter tube.. be specific please!!?
Answer:
I'm sorry I just don't recall the physics to be any more specific, perhaps you could repost your question there to get a better response, but in short your optical rotation will not be accurate.
The light that is passing through your sample is passing from sample to air and then back to sample, each with a different refractive index, and this will effect how the light is bent each time it enters and exits one of these regions.
As for the polarization, well that will be a function on the incident angle on the incoming wave, and if it is bent at different angles at differing points throught the sample width, I would think that the optical rotation would be off and very unreproducible.
I wouldn't be surprised if you ran one sample and got, say, +22.2º, another and got +15.1º and then a third and got +33.2º.
Again, the physicists may be able to answer this better - it might be better to ask them.
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